Biochemical Studies of Virus Reproduction I. Purification and Properties of Escherichia Coli
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چکیده
Bacteriophages possess the fundamental properties of viruses, yet exhibit ease of assay, handling, and culture, and thus present suitable systems for isotope tracer studies of the mode of virus reproduction and protein synthesis. In an investigation of this problem we have employed the Escherichia coli bacteriophage Ta system. This communication outlines methods for the growth and isolation of this phage and describes the properties and physicochemical characterization of the purified virus. A following paper’ discusses the chemical composition of bacteriophage Ts. Later articles will cover the application of isotope indicators to the study of the biological precursors of virus nucleic acid and protein. A preliminary report on the origin of virus phosphorus has already been published (2). Numerous biological studies on the bacteriophages attacking E. coli have recently appeared. The seven types of coliphages most commonly studied (Tl, Tz, . . .TT) were first described by Demerec and Fano (3). Their biological properties and their differentiation on the basis of plaque type, morphology, size, antigenic structure, and host range have been reviewed by Delbrtick (4, 5). Although electron micrographs of several of these coliphages have been published (6-8), physicochemical characterization has thus far been meager. Only bacteriophage TZ has previously been isolated in quantity and characterized in the ultracentrifuge (8, 9), and electrophoretic diagrams have not yet been published for any of the bacterial viruses.
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تاریخ انتشار 2000